› 1k8c

X-ray diffraction
2.1Å resolution

Crystal structure of dimeric xylose reductase in complex with NADP(H)

Released:
DOI: 10.2210/pdb1k8c/pdb
PDB entry 1k8c coloured by chain, front view.
PDB entry 1k8c coloured by chain, side view.
PDB entry 1k8c coloured by chain, top view.
Source organism: Yamadazyma tenuis
Primary publication:
The structure of apo and holo forms of xylose reductase, a dimeric aldo-keto reductase from Candida tenuis.
Kavanagh KL, Klimacek M, Nidetzky B, Wilson DK
Biochemistry 41 8785-95 (2002)
PMID: 12102621

Function and Biology Details

Reaction catalysed: 
Xylitol + NAD(P)(+) = D-xylose + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-130775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(P)H-dependent D-xylose reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 322 amino acids
Theoretical weight: 36.06 KDa
Source organism: Yamadazyma tenuis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O74237 (Residues: 1-322; Coverage: 100%)
Gene names: XYL1, XYLR
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 182.202Å b: 127.809Å c: 80.251Å
α: 90° β: 90.37° γ: 90°
R-values:
R R work R free
0.213 0.213 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives.
Matsushika et al. (2009)
3 more

9 mentions without citation

Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.
Woodyer et al. (2005)
8 more