› 1k8g

X-ray diffraction
2.6Å resolution

Crystal Structure of the N-terminal domain of Oxytricha nova telomere end binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA

Released:
DOI: 10.2210/pdb1k8g/pdb
PDB entry 1k8g coloured by chain, front view.
PDB entry 1k8g coloured by chain, side view.
PDB entry 1k8g coloured by chain, top view.
Source organism: Sterkiella nova
Primary publication:
Crystal structure of the N-terminal domain of Oxytricha nova telomere end-binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA.
Classen S, Ruggles JA, Schultz SC
J Mol Biol 314 1113-25 (2001)
PMID: 11743727

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-114060 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Telomere-binding protein subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 320 amino acids
Theoretical weight: 36.36 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P29549 (Residues: 1-320; Coverage: 65%)
Gene names: MAC-56A, MAC-56K, MAC-56S
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
5'-D(TP*TP*GP*GP*GP*G)-3' Chains: D, E
Molecule details ›
Chains: D, E
Length: 6 nucleotides
Theoretical weight: 1.88 KDa
Source organism: Sterkiella nova
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 121.182Å b: 121.182Å c: 137.169Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.246 0.246 0.285
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Nucleic acid recognition by OB-fold proteins.
Theobald et al. (2003)
4 more

5 mentions without citation

Structural anatomy of telomere OB proteins.
Horvath MP. (2011)
4 more