› 1k8t

X-ray diffraction
2.6Å resolution

Crystal structure of the adenylyl cyclase domain of anthrax edema factor (EF)

Released:
DOI: 10.2210/pdb1k8t/pdb
PDB entry 1k8t coloured by chain, front view.
PDB entry 1k8t coloured by chain, side view.
PDB entry 1k8t coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
Structural basis for the activation of anthrax adenylyl cyclase exotoxin by calmodulin.
Drum CL, Yan SZ, Bard J, Shen YQ, Lu D, Soelaiman S, Grabarek Z, Bohm A, Tang WJ
Nature 415 396-402 (2002)
PMID: 11807546

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-154036 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin-sensitive adenylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 510 amino acids
Theoretical weight: 58.81 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P40136 (Residues: 291-800; Coverage: 67%)
Gene names: BXA0141, GBAA_pXO1_0142, cya, pXO1-122
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P21212
Unit cell:
a: 50.476Å b: 203.604Å c: 74.034Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.276
Expression system: Escherichia coli BL21

Quick links

Citations

57 review citations

Calmodulin in action: diversity in target recognition and activation mechanisms.
Hoeflich et al. (2002)
56 more

12 mentions without citation

The adenylyl cyclase activity of anthrax edema factor.
Tang et al. (2009)
11 more