› 1k9y

X-ray diffraction
1.9Å resolution

The PAPase Hal2p complexed with magnesium ions and reaction products: AMP and inorganic phosphate

Released:
DOI: 10.2210/pdb1k9y/pdb
PDB entry 1k9y coloured by chain, front view.
PDB entry 1k9y coloured by chain, side view.
PDB entry 1k9y coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural enzymology of Li(+)-sensitive/Mg(2+)-dependent phosphatases.
Patel S, Martínez-Ripoll M, Blundell TL, Albert A
J Mol Biol 320 1087-94 (2002)
PMID: 12126627

Function and Biology Details

Reaction catalysed: 
Adenosine 3',5'-bisphosphate + H(2)O = adenosine 5'-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152225 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3'(2'),5'-bisphosphate nucleotidase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 39.2 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P32179 (Residues: 1-357; Coverage: 100%)
Gene names: HAL2, MET22, YOL064C
Sequence domains: Inositol monophosphatase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand PO4 1 x PO4
Ligand AMP 1 x AMP
Ligand BME 1 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 54.693Å b: 45.075Å c: 71.794Å
α: 90° β: 110.96° γ: 90°
R-values:
R R work R free
0.158 0.156 0.203
Expression system: Saccharomyces cerevisiae

Quick links

Citations

5 review citations

Drugs, their targets and the nature and number of drug targets.
Imming et al. (2006)
4 more

2 mentions without citation

An Ancient Fingerprint Indicates the Common Ancestry of Rossmann-Fold Enzymes Utilizing Different Ribose-Based Cofactors.
Laurino et al. (2016)
1 more