› 1kaf

X-ray diffraction
1.6Å resolution

DNA Binding Domain Of The Phage T4 Transcription Factor MotA (AA105-211)

Released:
DOI: 10.2210/pdb1kaf/pdb
PDB entry 1kaf coloured by chain, front view.
PDB entry 1kaf coloured by chain, side view.
PDB entry 1kaf coloured by chain, top view.
Source organism: Escherichia virus T4
Primary publication:
The MotA transcription factor from bacteriophage T4 contains a novel DNA-binding domain: the 'double wing' motif.
Li N, Sickmier EA, Zhang R, Joachimiak A, White SW
Mol Microbiol 43 1079-88 (2002)
PMID: 11918797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Middle transcription regulatory protein motA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 108 amino acids
Theoretical weight: 12.54 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P22915 (Residues: 105-211; Coverage: 51%)
Gene name: motA
Sequence domains: Bacteriophage T4 MotA, C-terminal
Structure domains: Transcription regulator MotA, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 31.284Å b: 54.733Å c: 91.445Å
α: 94.77° β: 91.1° γ: 95.61°
R-values:
R R work R free
0.226 0.226 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Transcriptional control in the prereplicative phase of T4 development.
Hinton DM. (2010)
1 more

6 mentions without citation

Architecture of the bacteriophage T4 activator MotA/promoter DNA interaction during sigma appropriation.
Hsieh et al. (2013)
5 more