› 1kcl

X-ray diffraction
1.94Å resolution

Bacillus ciruclans strain 251 Cyclodextrin glycosyl transferase mutant G179L

Released:
DOI: 10.2210/pdb1kcl/pdb
PDB entry 1kcl coloured by chain, front view.
PDB entry 1kcl coloured by chain, side view.
PDB entry 1kcl coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
The remote substrate binding subsite -6 in cyclodextrin-glycosyltransferase controls the transferase activity of the enzyme via an induced-fit mechanism.
Leemhuis H, Uitdehaag JC, Rozeboom HJ, Dijkstra BW, Dijkhuizen L
J Biol Chem 277 1113-9 (2002)
PMID: 11696539

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.63 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC, BGC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand CA 2 x CA
Ligand GLC 1 x GLC
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 114.714Å b: 109.473Å c: 65.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.171 0.19
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.
Leemhuis et al. (2010)
2 more

3 mentions without citation

Hyaline fibromatosis syndrome inducing mutations in the ectodomain of anthrax toxin receptor 2 can be rescued by proteasome inhibitors.
Deuquet et al. (2011)
2 more