› 1kcm

X-ray diffraction
2Å resolution

Crystal Structure of Mouse PITP Alpha Void of Bound Phospholipid at 2.0 Angstroms Resolution

Released:
DOI: 10.2210/pdb1kcm/pdb
PDB entry 1kcm coloured by chain, front view.
PDB entry 1kcm coloured by chain, side view.
PDB entry 1kcm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of apo-phosphatidylinositol transfer protein alpha provides insight into membrane association.
Schouten A, Agianian B, Westerman J, Kroon J, Wirtz KW, Gros P
EMBO J 21 2117-21 (2002)
PMID: 11980708

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol transfer protein alpha isoform Chain: A
Molecule details ›
Chain: A
Length: 270 amino acids
Theoretical weight: 31.81 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53810 (Residues: 2-271; Coverage: 100%)
Gene names: Pitpn, Pitpna
Sequence domains: Phosphatidylinositol transfer protein
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 50.464Å b: 50.464Å c: 216.105Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.216 0.273
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
20 more

10 mentions without citation

Inositol lipid regulation of lipid transfer in specialized membrane domains.
Kim et al. (2013)
9 more