› 1kea

X-ray diffraction
2Å resolution

STRUCTURE OF A THERMOSTABLE THYMINE-DNA GLYCOSYLASE

Released:
DOI: 10.2210/pdb1kea/pdb
PDB entry 1kea coloured by chain, front view.
PDB entry 1kea coloured by chain, side view.
PDB entry 1kea coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
Structure and activity of a thermostable thymine-DNA glycosylase: evidence for base twisting to remove mismatched normal DNA bases.
Mol CD, Arvai AS, Begley TJ, Cunningham RP, Tainer JA
J Mol Biol 315 373-84 (2002)
PMID: 11786018

Function and Biology Details

Reaction catalysed: 
Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-151542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymine/uracil-DNA glycosylase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 25.44 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29588 (Residues: 1-221; Coverage: 100%)
Gene name: mig
Sequence domains: HhH-GPD superfamily base excision DNA repair protein
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 1 x ZN
Ligand ACT 4 x ACT
Ligand CL 2 x CL
Ligand SF4 1 x SF4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P41212
Unit cell:
a: 68.215Å b: 68.215Å c: 98.944Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.211 0.248
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Uracil in DNA--occurrence, consequences and repair.
Krokan et al. (2002)
9 more

4 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
3 more