› 1keb

X-ray diffraction
1.8Å resolution

Crystal Structure of Double Mutant M37L,P40S E.coli Thioredoxin

Released:
DOI: 10.2210/pdb1keb/pdb
PDB entry 1keb coloured by chain, front view.
PDB entry 1keb coloured by chain, side view.
PDB entry 1keb coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural consequences of replacement of an alpha-helical Pro residue in Escherichia coli thioredoxin.
Jain R, Dani V, Mitra A, Srivastava S, Sarma SP, Varadarajan R, Ramakumar S
Protein Eng 15 627-33 (2002)
PMID: 12364576

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141959 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 108 amino acids
Theoretical weight: 11.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P1
Unit cell:
a: 29.32Å b: 37.5Å c: 50.74Å
α: 69.25° β: 79.71° γ: 85.39°
R-values:
R R work R free
0.182 0.182 0.222
Expression system: Escherichia coli BL21(DE3)

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Citations

10 citation in other articles

Native Proteomics in Discovery Mode Using Size-Exclusion Chromatography-Capillary Zone Electrophoresis-Tandem Mass Spectrometry.
Shen et al. (2018)
9 more

3 mentions without citation

Designing of peptide aptamer targeting the receptor-binding domain of spike protein of SARS-CoV-2: an in silico study.
Devi et al. (2022)
2 more