› 1kfa

X-ray diffraction
2.8Å resolution

Crystal structure of Fab fragment complexed with gibberellin A4

Released:
DOI: 10.2210/pdb1kfa/pdb
PDB entry 1kfa coloured by chain, front view.
PDB entry 1kfa coloured by chain, side view.
PDB entry 1kfa coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the liganded anti-gibberellin A(4) antibody 4-B8(8)/E9 Fab fragment.
Murata T, Fushinobu S, Nakajima M, Asami O, Sassa T, Wakagi T, Yamaguchi I
Biochem Biophys Res Commun 293 489-96 (2002)
PMID: 12054627

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-233706 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
monoclonal antibody light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 217 amino acids
Theoretical weight: 23.75 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
monoclonal antibody heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 221 amino acids
Theoretical weight: 23.76 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GA4 2 x GA4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: C2221
Unit cell:
a: 118.076Å b: 150.146Å c: 106.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.235 0.313
Expression system: Mus musculus

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Citations

1 review citation

Plant hormone binding sites.
Napier R. (2004)
7 other articles

2 mentions without citation

Revealing novel telomere proteins using in vivo cross-linking, tandem affinity purification, and label-free quantitative LC-FTICR-MS.
Nittis et al. (2010)
1 more