› 1kfg

X-ray diffraction
1.9Å resolution

The X-ray Crystal Structure of Cel9G from Clostridium cellulolyticum complexed with a Thio-Oligosaccharide Inhibitor

Released:
DOI: 10.2210/pdb1kfg/pdb
PDB entry 1kfg coloured by chain, front view.
PDB entry 1kfg coloured by chain, side view.
PDB entry 1kfg coloured by chain, top view.
Source organism: Ruminiclostridium cellulolyticum
Primary publication:
X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Mandelman D, Belaich A, Belaich JP, Aghajari N, Driguez H, Haser R
J Bacteriol 185 4127-35 (2003)
PMID: 12837787

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endoglucanase G Chains: A, B
Molecule details ›
Chains: A, B
Length: 614 amino acids
Theoretical weight: 68.06 KDa
Source organism: Ruminiclostridium cellulolyticum
Expression system: Escherichia coli
UniProt:
  • Canonical: P37700 (Residues: 36-649; Coverage: 89%)
Gene names: Ccel_0731, celCCG
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GS1, SGC, BGC
Ligand GS1 2 x GS1
Ligand SGC 4 x SGC
Ligand BGC 2 x BGC
5 bound ligands:
Ligand CA 4 x CA
Ligand MG 5 x MG
Ligand TRS 1 x TRS
Ligand GOL 4 x GOL
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P1
Unit cell:
a: 57.04Å b: 57.73Å c: 86.63Å
α: 93.86° β: 100.83° γ: 99.54°
R-values:
R R work R free
0.167 0.167 0.204
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia.
Desvaux M. (2005)
3 more

4 mentions without citation

X-Ray crystal structure of the multidomain endoglucanase Cel9G from Clostridium cellulolyticum complexed with natural and synthetic cello-oligosaccharides.
Mandelman et al. (2003)
3 more