› 1kfw

X-ray diffraction
1.74Å resolution

Structure of catalytic domain of psychrophilic chitinase B from Arthrobacter TAD20

Released:
DOI: 10.2210/pdb1kfw/pdb
PDB entry 1kfw coloured by chain, front view.
PDB entry 1kfw coloured by chain, side view.
PDB entry 1kfw coloured by chain, top view.
Source organism: Arthrobacter sp. TAD20
Entry authors: Ayati M, Mandelman D, Aghajari N, Haser R

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 435 amino acids
Theoretical weight: 46.49 KDa
Source organism: Arthrobacter sp. TAD20
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9REI6 (Residues: 48-482; Coverage: 81%)
Gene name: chitiB
Sequence domains: Glycosyl hydrolases family 18
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GOL 5 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P3221
Unit cell:
a: 114.28Å b: 114.28Å c: 102.78Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.178 0.174 0.189
Expression system: Escherichia coli

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