› 1khc

X-ray diffraction
1.8Å resolution

Crystal Structure of the PWWP Domain of Mammalian DNA Methyltransferase Dnmt3b

Released:
DOI: 10.2210/pdb1khc/pdb
PDB entry 1khc coloured by chain, front view.
PDB entry 1khc coloured by chain, side view.
PDB entry 1khc coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The PWWP domain of mammalian DNA methyltransferase Dnmt3b defines a new family of DNA-binding folds.
Qiu C, Sawada K, Zhang X, Cheng X
Nat Struct Biol 9 217-24 (2002)
PMID: 11836534

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131548 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA (cytosine-5)-methyltransferase 3B Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.67 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O88509 (Residues: 219-362; Coverage: 17%)
Gene name: Dnmt3b
Sequence domains: PWWP domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UNX 22 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6522
Unit cell:
a: 52.75Å b: 52.75Å c: 186.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.19 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

43 review citations

Genomic DNA methylation: the mark and its mediators.
Klose et al. (2006)
42 more

7 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
6 more