› 1kjy

X-ray diffraction
2.7Å resolution

Crystal Structure of Human G[alpha]i1 Bound to the GoLoco Motif of RGS14

Released:
DOI: 10.2210/pdb1kjy/pdb
PDB entry 1kjy coloured by chain, front view.
PDB entry 1kjy coloured by chain, side view.
PDB entry 1kjy coloured by chain, top view.
Source organisms:
Primary publication:
Structural determinants for GoLoco-induced inhibition of nucleotide release by Galpha subunits.
Kimple RJ, Kimple ME, Betts L, Sondek J, Siderovski DP
Nature 416 878-81 (2002)
PMID: 11976690

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-126668 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 325 amino acids
Theoretical weight: 37.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63096 (Residues: 30-354; Coverage: 92%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 14 Chains: B, D
Molecule details ›
Chains: B, D
Length: 36 amino acids
Theoretical weight: 4.09 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: O08773 (Residues: 496-531; Coverage: 7%)
Gene name: Rgs14
Sequence domains: GoLoco motif

Ligands and Environments

3 bound ligands:
Ligand CS 15 x CS
Ligand GDP 2 x GDP
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P2221
Unit cell:
a: 69.51Å b: 82.04Å c: 187.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.299
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

37 review citations

The GAPs, GEFs, and GDIs of heterotrimeric G-protein alpha subunits.
Siderovski et al. (2005)
36 more

18 mentions without citation

Overview of protein structural and functional folds.
Sun et al. (2004)
17 more