› 1kk8

X-ray diffraction
2.3Å resolution

SCALLOP MYOSIN (S1-ADP-BeFx) IN THE ACTIN-DETACHED CONFORMATION

Released:
DOI: 10.2210/pdb1kk8/pdb
PDB entry 1kk8 coloured by chain, front view.
PDB entry 1kk8 coloured by chain, side view.
PDB entry 1kk8 coloured by chain, top view.
Source organism: Argopecten irradians
Primary publication:
Crystallographic findings on the internally uncoupled and near-rigor states of myosin: further insights into the mechanics of the motor.
Himmel DM, Gourinath S, Reshetnikova L, Shen Y, Szent-Györgyi AG, Cohen C
Proc Natl Acad Sci U S A 99 12645-50 (2002)
PMID: 12297624

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139482 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin heavy chain, striated muscle Chain: A
Molecule details ›
Chain: A
Length: 837 amino acids
Theoretical weight: 95.54 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P24733 (Residues: 1-837; Coverage: 43%)
Sequence domains:
Myosin regulatory light chain, striated adductor muscle Chain: B
Molecule details ›
Chain: B
Length: 139 amino acids
Theoretical weight: 15.89 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P13543 (Residues: 14-152; Coverage: 89%)
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Myosin essential light chain, striated adductor muscle Chain: C
Molecule details ›
Chain: C
Length: 154 amino acids
Theoretical weight: 17.39 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P07291 (Residues: 2-155; Coverage: 98%)
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand BEF 1 x BEF
Ligand ADP 1 x ADP
Ligand GOL 1 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P1
Unit cell:
a: 51.604Å b: 58.527Å c: 133.295Å
α: 81.08° β: 84.94° γ: 67.24°
R-values:
R R work R free
0.234 0.23 0.269

Quick links

Citations

7 review citations

SH3 domains: modules of protein-protein interactions.
Kurochkina et al. (2013)
6 more

37 mentions without citation

Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.
Spudich JA. (2019)
36 more