› 1kl7

X-ray diffraction
2.7Å resolution

Crystal Structure of Threonine Synthase from Yeast

Released:
DOI: 10.2210/pdb1kl7/pdb
PDB entry 1kl7 coloured by chain, front view.
PDB entry 1kl7 coloured by chain, side view.
PDB entry 1kl7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and function of threonine synthase from yeast.
Garrido-Franco M, Ehlert S, Messerschmidt A, Marinkovic' S, Huber R, Laber B, Bourenkov GP, Clausen T
J. Biol. Chem. 277 12396-405 (2002)
PMID: 11756443

Function and Biology Details

Reaction catalysed: 
O-phospho-L-homoserine + H(2)O = L-threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147630 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 514 amino acids
Theoretical weight: 57.87 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P16120 (Residues: 1-514; Coverage: 100%)
Gene names: THR4, YCR053W, YCR53W
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 95.3Å b: 51.6Å c: 106.9Å
α: 90° β: 99.6° γ: 90°
R-values:
R R work R free
0.201 0.201 0.253
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The aspartic acid metabolic pathway, an exciting and essential pathway in plants.
Azevedo et al. (2006)
4 more

4 mentions without citation

The protein common interface database (ProtCID)--a comprehensive database of interactions of homologous proteins in multiple crystal forms.
Xu et al. (2011)
3 more