› 1kln

X-ray diffraction
3.2Å resolution

DNA POLYMERASE I KLENOW FRAGMENT (E.C.2.7.7.7) MUTANT/DNA COMPLEX

Released:
DOI: 10.2210/pdb1kln/pdb
PDB entry 1kln coloured by chain, front view.
PDB entry 1kln coloured by chain, side view.
PDB entry 1kln coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of DNA polymerase I Klenow fragment bound to duplex DNA.
Beese LS, Derbyshire V, Steitz TA
Science 260 352-5 (1993)
PMID: 8469987

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114086 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 605 amino acids
Theoretical weight: 68.12 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00582 (Residues: 324-928; Coverage: 65%)
Gene names: JW3835, b3863, polA, resA
Sequence domains:
Structure domains:
DNA (5'-D(*GP*CP*CP*TP*CP*GP*CP*GP*GP*CP*GP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*GP*CP*CP*GP*CP*GP*AP*GP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.07 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43
Unit cell:
a: 104.3Å b: 104.3Å c: 86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 not available
Expression system: Not provided

Quick links

Citations

39 review citations

DNA replication fidelity.
Kunkel et al. (2000)
38 more

34 mentions without citation

Genetic constraints on protein evolution.
Camps et al. (2007)
33 more