› 1kme

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF BACTERIORHODOPSIN CRYSTALLIZED FROM BICELLES

Released:
DOI: 10.2210/pdb1kme/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1kme coloured by chain, front view.
PDB entry 1kme coloured by chain, side view.
PDB entry 1kme coloured by chain, top view.
Source organism: Halobacterium salinarum
Primary publication:
Bicelle crystallization: a new method for crystallizing membrane proteins yields a monomeric bacteriorhodopsin structure.
Faham S, Bowie JU
J Mol Biol 316 1-6 (2002)
PMID: 11829498

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 25.3 KDa
Source organism: Halobacterium salinarum
UniProt:
  • Canonical: P02945 (Residues: 14-244; Coverage: 88%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 2 x RET
Ligand SQU 2 x SQU
Ligand BGC 2 x BGC
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 45Å b: 108.9Å c: 55.9Å
α: 90° β: 113.58° γ: 90°
R-values:
R R work R free
0.263 0.263 0.275

Quick links

Citations

46 review citations

The structure and function of G-protein-coupled receptors.
Rosenbaum et al. (2009)
45 more

10 mentions without citation

Interdisciplinary biophysical studies of membrane proteins bacteriorhodopsin and rhodopsin.
Fahmy et al. (2023)
9 more