› 1koa

X-ray diffraction
3.3Å resolution

TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS

Released:
DOI: 10.2210/pdb1koa/pdb
PDB entry 1koa coloured by chain, front view.
PDB entry 1koa coloured by chain, side view.
PDB entry 1koa coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Giant protein kinases: domain interactions and structural basis of autoregulation.
Kobe B, Heierhorst J, Feil SC, Parker MW, Benian GM, Weiss KR, Kemp BE
EMBO J 15 6810-21 (1996)
PMID: 9003756

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Twitchin Chain: A
Molecule details ›
Chain: A
Length: 491 amino acids
Theoretical weight: 56.43 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q23551 (Residues: 6209-6699; Coverage: 7%)
Gene names: ZK617.1, unc-22
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: I213
Unit cell:
a: 195.5Å b: 195.5Å c: 195.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.351
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Regulation of protein kinases; controlling activity through activation segment conformation.
Nolen et al. (2004)
16 more

14 mentions without citation

Cytoskeletal protein kinases: titin and its relations in mechanosensing.
Gautel M. (2011)
13 more