› 1kp9

X-ray diffraction
2.21Å resolution

Crystal structure of mycolic acid cyclopropane synthase CmaA1, apo-form

Released:
DOI: 10.2210/pdb1kp9/pdb
PDB entry 1kp9 coloured by chain, front view.
PDB entry 1kp9 coloured by chain, side view.
PDB entry 1kp9 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis.
Huang CC, Smith CV, Glickman MS, Jacobs WR, Sacchettini JC
J Biol Chem 277 11559-69 (2002)
PMID: 11756461

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161940 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclopropane mycolic acid synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 32.5 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPB7 (Residues: 1-287; Coverage: 100%)
Gene names: CMAS-1, MTV004.50, Rv3392c, cma1, cmaA1
Sequence domains: Mycolic acid cyclopropane synthetase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand ACY 3 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 50.342Å b: 73.812Å c: 76.043Å
α: 90° β: 107.07° γ: 90°
R-values:
R R work R free
0.2 0.2 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Antituberculosis drugs: ten years of research.
Janin YL. (2007)
22 more

5 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
4 more