› 1kpf

X-ray diffraction
1.5Å resolution

PKCI-SUBSTRATE ANALOG

Released:
DOI: 10.2210/pdb1kpf/pdb
PDB entry 1kpf coloured by chain, front view.
PDB entry 1kpf coloured by chain, side view.
PDB entry 1kpf coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based analysis of catalysis and substrate definition in the HIT protein family.
Lima CD, Klein MG, Hendrickson WA
Science 278 286-90 (1997)
PMID: 9323207

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156024 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine 5'-monophosphoramidase HINT1 Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P49773 (Residues: 2-126; Coverage: 99%)
Gene names: HINT, HINT1, PKCI1, PRKCNH1
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

1 bound ligand:
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 40.15Å b: 40.15Å c: 143.19Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.24
Expression system: Komagataella pastoris

Quick links

Citations

29 review citations

Messenger RNA turnover in eukaryotes: pathways and enzymes.
Meyer et al. (2004)
28 more

21 mentions without citation

Evaluating conformational free energies: the colony energy and its application to the problem of loop prediction.
Xiang et al. (2002)
20 more