› 1kpk

X-ray diffraction
3.5Å resolution

Crystal Structure of the ClC Chloride Channel from E. coli

Released:
DOI: 10.2210/pdb1kpk/pdb
PDB entry 1kpk coloured by chain, front view.
PDB entry 1kpk coloured by chain, side view.
PDB entry 1kpk coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
X-ray structure of a ClC chloride channel at 3.0 A reveals the molecular basis of anion selectivity.
Dutzler R, Campbell EB, Cadene M, Chait BT, MacKinnon R
Nature 415 287-94 (2002)
PMID: 11796999

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
H(+)/Cl(-) exchange transporter ClcA Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 473 amino acids
Theoretical weight: 50.39 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37019 (Residues: 1-473; Coverage: 100%)
Gene names: JW5012, b0155, clcA, eriC, yadQ
Sequence domains: Voltage gated chloride channel
Structure domains: Clc chloride channel

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P212121
Unit cell:
a: 105.66Å b: 152.525Å c: 263.064Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.291 0.29 0.301
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

232 review citations

Lysosomal acidification mechanisms.
Mindell JA. (2012)
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20 mentions without citation

Inverted topologies in membrane proteins: a mini-review.
Duran et al. (2013)
19 more