› 1kq4

X-ray diffraction
2.25Å resolution

CRYSTAL STRUCTURE OF A THY1-COMPLEMENTING PROTEIN (TM0449) FROM THERMOTOGA MARITIMA AT 2.25 A RESOLUTION

Released:
DOI: 10.2210/pdb1kq4/pdb
PDB entry 1kq4 coloured by chain, front view.
PDB entry 1kq4 coloured by chain, side view.
PDB entry 1kq4 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline.
Lesley SA, Kuhn P, Godzik A, Deacon AM, Mathews I, Kreusch A, Spraggon G, Klock HE, McMullan D, Shin T, Vincent J, Robb A, Brinen LS, Miller MD, McPhillips TM, Miller MA, Scheibe D, Canaves JM, Guda C, Jaroszewski L, Selby TL, Elsliger MA, Wooley J, Taylor SS, Hodgson KO, Wilson IA, Schultz PG, Stevens RC
Proc Natl Acad Sci U S A 99 11664-9 (2002)
PMID: 12193646

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavin-dependent thymidylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 232 amino acids
Theoretical weight: 27.79 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYT0 (Residues: 1-220; Coverage: 100%)
Gene names: TM_0449, thy1, thyX
Sequence domains: Thymidylate synthase complementing protein
Structure domains: Gyrase A; domain 2

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
No bound ligands
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 54.2Å b: 116.61Å c: 141.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.192 0.24
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Protein production and purification.
Structural Genomics et al. (2008)
27 more

2 mentions without citation

Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline.
Lesley et al. (2002)
1 more