› 1ks2

X-ray diffraction
1.5Å resolution

Crystal Structure Analysis of the rpiA, Structural Genomics, protein EC1268.

Released:
DOI: 10.2210/pdb1ks2/pdb
PDB entry 1ks2 coloured by chain, front view.
PDB entry 1ks2 coloured by chain, side view.
PDB entry 1ks2 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of Escherichia coli ribose-5-phosphate isomerase: a ubiquitous enzyme of the pentose phosphate pathway and the Calvin cycle.
Zhang Rg, Andersson CE, Savchenko A, Skarina T, Evdokimova E, Beasley S, Arrowsmith CH, Edwards AM, Joachimiak A, Mowbray SL
Structure 11 31-42 (2003)
PMID: 12517338

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 23.16 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z0 (Residues: 1-219; Coverage: 100%)
Gene names: JW5475, b2914, rpiA, ygfC
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P1
Unit cell:
a: 42.119Å b: 42.12Å c: 59.429Å
α: 89.94° β: 100.95° γ: 98.73°
R-values:
R R work R free
0.224 0.224 0.237
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The many faces of the helix-turn-helix domain: transcription regulation and beyond.
Aravind et al. (2005)
11 more

3 mentions without citation

Structure of Escherichia coli ribose-5-phosphate isomerase: a ubiquitous enzyme of the pentose phosphate pathway and the Calvin cycle.
Zhang et al. (2003)
2 more