› 1ksi

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF A EUKARYOTIC (PEA SEEDLING) COPPER-CONTAINING AMINE OXIDASE AT 2.2A RESOLUTION

Released:
DOI: 10.2210/pdb1ksi/pdb
PDB entry 1ksi coloured by chain, front view.
PDB entry 1ksi coloured by chain, side view.
PDB entry 1ksi coloured by chain, top view.
Source organism: Pisum sativum
Primary publication:
Crystal structure of a eukaryotic (pea seedling) copper-containing amine oxidase at 2.2 A resolution.
Kumar V, Dooley DM, Freeman HC, Guss JM, Harvey I, McGuirl MA, Wilce MC, Zubak VM
Structure 4 943-55 (1996)
PMID: 8805580

Function and Biology Details

Reaction catalysed: 
RCH(2)NH(2) + H(2)O + O(2) = RCHO + NH(3) + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174980 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Primary amine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 642 amino acids
Theoretical weight: 73.05 KDa
Source organism: Pisum sativum
UniProt:
  • Canonical: Q43077 (Residues: 31-672; Coverage: 99%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
3 bound ligands:
Ligand NAG 2 x NAG
Ligand CU 2 x CU
Ligand MN 2 x MN
1 modified residue:
Ligand TPQ 2 x TPQ

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 85.37Å b: 114.64Å c: 199.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 not available

Quick links

Citations

16 review citations

Copper homeostasis.
Burkhead et al. (2009)
15 more

9 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
8 more