Function and Biology Details
Reaction catalysed:
Endohydrolysis of 1,4-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose-2-sulfate in iota-carrageenans
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Iota-carrageenase (preferred)
PDBe Complex ID:
PDB-CPX-190254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Iota-carrageenase
Molecule details ›
Chains: A, B
Length: 464 amino acids
Theoretical weight: 51.94 KDa
Source organism: Alteromonas macleodii
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Pectate lyase superfamily protein
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like
Length: 464 amino acids
Theoretical weight: 51.94 KDa
Source organism: Alteromonas macleodii
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q9F5I8 (Residues: 28-491; Coverage: 99%)
Sequence domains: Pectate lyase superfamily protein
Structure domains: Single-stranded right-handed beta-helix, Pectin lyase-like
Ligands and Environments
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE BM30A
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)
