› 1ku0

X-ray diffraction
2Å resolution

Structure of the Bacillus stearothermophilus L1 lipase

Released:
DOI: 10.2210/pdb1ku0/pdb
PDB entry 1ku0 coloured by chain, front view.
PDB entry 1ku0 coloured by chain, side view.
PDB entry 1ku0 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Novel zinc-binding center and a temperature switch in the Bacillus stearothermophilus L1 lipase.
Jeong ST, Kim HK, Kim SJ, Chi SW, Pan JG, Oh TK, Ryu SE
J Biol Chem 277 17041-7 (2002)
PMID: 11859083

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130292 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.19 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66015 (Residues: 30-417; Coverage: 93%)
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 119.62Å b: 85.04Å c: 98.36Å
α: 90° β: 99.73° γ: 90°
R-values:
R R work R free
0.185 0.185 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
1 more

10 mentions without citation

Application of a sensitive collection heuristic for very large protein families: evolutionary relationship between adipose triglyceride lipase (ATGL) and classic mammalian lipases.
Schneider et al. (2006)
9 more