› 1ku2

X-ray diffraction
2.9Å resolution

Crystal Structure of Thermus aquaticus RNA Polymerase Sigma Subunit Fragment Containing Regions 1.2 to 3.1

Released:
DOI: 10.2210/pdb1ku2/pdb
PDB entry 1ku2 coloured by chain, front view.
PDB entry 1ku2 coloured by chain, side view.
PDB entry 1ku2 coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Structure of the bacterial RNA polymerase promoter specificity sigma subunit.
Campbell EA, Muzzin O, Chlenov M, Sun JL, Olson CA, Weinman O, Trester-Zedlitz ML, Darst SA
Mol Cell 9 527-39 (2002)
PMID: 11931761

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190230 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA polymerase sigma factor SigA Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.61 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EZJ8 (Residues: 92-332; Coverage: 55%)
Gene name: sigA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P65
Unit cell:
a: 104.186Å b: 104.186Å c: 169.181Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.23 0.23 0.271
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

53 review citations

Multiple sigma subunits and the partitioning of bacterial transcription space.
Gruber et al. (2003)
52 more

5 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
4 more