› 1kx0

X-ray diffraction
2Å resolution

Rat mannose protein A (H189V I207V) complexed with man-a13-man

Released:
DOI: 10.2210/pdb1kx0/pdb
PDB entry 1kx0 coloured by chain, front view.
PDB entry 1kx0 coloured by chain, side view.
PDB entry 1kx0 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Orientation of bound ligands in mannose-binding proteins. Implications for multivalent ligand recognition.
Ng KK, Kolatkar AR, Park-Snyder S, Feinberg H, Clark DA, Drickamer K, Weis WI
J Biol Chem 277 16088-95 (2002)
PMID: 11850428

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Mannose-binding protein A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 149 amino acids
Theoretical weight: 16.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19999 (Residues: 90-238; Coverage: 67%)
Gene name: Mbl1
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
2 bound ligands:
Ligand CA 9 x CA
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 79.08Å b: 85.11Å c: 97.66Å
α: 90° β: 97.66° γ: 90°
R-values:
R R work R free
0.201 0.201 0.232
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The C-type lectin-like domain superfamily.
Zelensky et al. (2005)
13 more

1 mention without citation

Backrub-like backbone simulation recapitulates natural protein conformational variability and improves mutant side-chain prediction.
Smith et al. (2008)