› 1kyt

X-ray diffraction
1.7Å resolution

Crystal Structure of Thermoplasma acidophilum 0175 (APC014)

Released:
DOI: 10.2210/pdb1kyt/pdb
PDB entry 1kyt coloured by chain, front view.
PDB entry 1kyt coloured by chain, side view.
PDB entry 1kyt coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Entry authors: Kim Y, Joachimiak A, Edwards A, Xu X, Pennycooke M, Gu J, Cheung F, Christendat D, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
2-phosphoglycolate + H(2)O = glycolate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190900 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycolate phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 26.03 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HLQ2 (Residues: 1-224; Coverage: 100%)
Gene name: Ta0175
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, APS BEAMLINE 19-BM
Spacegroup: I222
Unit cell:
a: 88.526Å b: 99.316Å c: 113.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.246
Expression system: Escherichia coli

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