› 1kzl

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reaction catalysed: 
2 6,7-dimethyl-8-(1-D-ribityl)lumazine = riboflavin + 4-(1-D-ribitylamino)-5-amino-2,6-dihydroxypyrimidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin synthase Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 22.89 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y7P0 (Residues: 1-208; Coverage: 100%)
Gene names: SPCC1450.13c, rib5
Sequence domains: Lumazine binding domain
Structure domains: Elongation Factor Tu (Ef-tu); domain 3

Ligands and Environments

2 bound ligands:
Ligand HG 1 x HG
Ligand CRM 2 x CRM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P61
Unit cell:
a: 70.485Å b: 70.485Å c: 92.952Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.185 0.22
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
10 more

4 mentions without citation

Prediction of functional sites based on the fuzzy oil drop model.
Bryliński et al. (2007)
3 more