› 1kzn

X-ray diffraction
2.3Å resolution

Crystal Structure of E. coli 24kDa Domain in Complex with Clorobiocin

Released:
DOI: 10.2210/pdb1kzn/pdb
PDB entry 1kzn coloured by chain, front view.
PDB entry 1kzn coloured by chain, side view.
PDB entry 1kzn coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
DNA gyrase interaction with coumarin-based inhibitors: the role of the hydroxybenzoate isopentenyl moiety and the 5'-methyl group of the noviose.
Lafitte D, Lamour V, Tsvetkov PO, Makarov AA, Klich M, Deprez P, Moras D, Briand C, Gilli R
Biochemistry 41 7217-23 (2002)
PMID: 12044152

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142496 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA gyrase subunit B Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.59 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AES6 (Residues: 15-219; Coverage: 26%)
Gene names: JW5625, acrB, b3699, cou, gyrB, himB, hisU, nalC, parA, pcbA
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains: Histidine kinase-like ATPase, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand CBN 1 x CBN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P212121
Unit cell:
a: 40.714Å b: 47.629Å c: 111.599Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.267
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Signal transduction mediated by the Ras/Raf/MEK/ERK pathway from cytokine receptors to transcription factors: potential targeting for therapeutic intervention.
Chang et al. (2003)
7 more

75 mentions without citation

Thiazole Ring-A Biologically Active Scaffold.
Petrou et al. (2021)
74 more