1l0v

X-ray diffraction
3.3Å resolution

Quinol-Fumarate Reductase with Menaquinol Molecules

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
PDBe Complex ID:
PDB-CPX-132435 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Fumarate reductase flavoprotein subunit Chains: A, M
Molecule details ›
Chains: A, M
Length: 602 amino acids
Theoretical weight: 66.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00363 (Residues: 1-602; Coverage: 100%)
Gene names: JW4115, b4154, frdA
Sequence domains:
Structure domains:
Fumarate reductase iron-sulfur subunit Chains: B, N
Molecule details ›
Chains: B, N
Length: 243 amino acids
Theoretical weight: 27.02 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AC47 (Residues: 2-244; Coverage: 100%)
Gene names: JW4114, b4153, frdB
Sequence domains:
Structure domains:
Fumarate reductase subunit C Chains: C, O
Molecule details ›
Chains: C, O
Length: 130 amino acids
Theoretical weight: 14.9 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8Q0 (Residues: 2-131; Coverage: 99%)
Gene names: JW4113, b4152, frdC
Sequence domains: Fumarate reductase subunit C
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit
Fumarate reductase subunit D Chains: D, P
Molecule details ›
Chains: D, P
Length: 119 amino acids
Theoretical weight: 13.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8Q3 (Residues: 1-119; Coverage: 100%)
Gene names: JW4112, b4151, frdD
Sequence domains: Fumarate reductase subunit D
Structure domains: Fumarate reductase/succinate dehydrogenase, transmembrane subunit

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 96.59Å b: 138.09Å c: 275.25Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.29
Expression system: Escherichia coli