› 1l1m

Solution NMR

SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1

Released:
DOI: 10.2210/pdb1l1m/pdb
PDB entry 1l1m coloured by chain, ensemble of 20 models, front view.
PDB entry 1l1m coloured by chain, ensemble of 20 models, side view.
PDB entry 1l1m coloured by chain, ensemble of 20 models, top view.
Source organism: Escherichia coli
Primary publication:
Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain.
Kalodimos CG, Bonvin AM, Salinas RK, Wechselberger R, Boelens R, Kaptein R
EMBO J 21 2866-76 (2002)
PMID: 12065400

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114104 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lactose operon repressor Chains: A, B
Molecule details ›
Chains: A, B
Length: 62 amino acids
Theoretical weight: 6.82 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03023 (Residues: 1-62; Coverage: 17%)
Gene names: JW0336, b0345, lacI
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
5'-D(*GP*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*GP*GP*AP*TP*AP*AP*CP*AP*AP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 23 nucleotides
Theoretical weight: 7.14 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*AP*AP*AP*TP*TP*GP*TP*TP*AP*TP*CP*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*TP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 23 nucleotides
Theoretical weight: 6.97 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING FOLLOWED BY RESTRAINED MDR
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

8 review citations

The lac repressor.
Lewis M. (2005)
7 more

11 mentions without citation

The role of dynamic conformational ensembles in biomolecular recognition.
Boehr et al. (2009)
10 more