› 1l1y

X-ray diffraction
2.4Å resolution

The Crystal Structure and Catalytic Mechanism of Cellobiohydrolase CelS, the Major Enzymatic Component of the Clostridium thermocellum cellulosome

Released:
DOI: 10.2210/pdb1l1y/pdb
PDB entry 1l1y coloured by chain, front view.
PDB entry 1l1y coloured by chain, side view.
PDB entry 1l1y coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
The crystal structure and catalytic mechanism of cellobiohydrolase CelS, the major enzymatic component of the Clostridium thermocellum Cellulosome.
Guimarães BG, Souchon H, Lytle BL, David Wu JH, Alzari PM
J Mol Biol 320 587-96 (2002)
PMID: 12096911

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and similar substrates, releasing cellobiose from the reducing ends of the chains.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Cellulose 1,4-beta-cellobiosidase (reducing end) CelS Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 678 amino acids
Theoretical weight: 76.64 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2S5 (Residues: 1-678; Coverage: 91%)
Gene name: celS
Sequence domains: Glycosyl hydrolase family 48
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P212121
Unit cell:
a: 147.243Å b: 207.204Å c: 213.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.224
Expression system: Escherichia coli

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Citations

5 review citations

Cellulase, clostridia, and ethanol.
Demain et al. (2005)
4 more

14 mentions without citation

Towards A Novel Multi-Epitopes Chimeric Vaccine for Simulating Strong Immune Responses and Protection against Morganella morganii.
Ullah et al. (2021)
13 more