› 1l2d

X-ray diffraction
2Å resolution

MutM (Fpg)-DNA Estranged Guanine Mismatch Recognition Complex

Released:
DOI: 10.2210/pdb1l2d/pdb
PDB entry 1l2d coloured by chain, front view.
PDB entry 1l2d coloured by chain, side view.
PDB entry 1l2d coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Structural insights into lesion recognition and repair by the bacterial 8-oxoguanine DNA glycosylase MutM.
Fromme JC, Verdine GL
Nat Struct Biol 9 544-52 (2002)
PMID: 12055620

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-114110 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MutM Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 30.74 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
Structure domains:
5'-D(*AP*G*GP*TP*AP*GP*AP*CP*GP*TP*GP*GP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.99 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
5'-D(*TP*GP*C*GP*TP*CP*CP*AP*(HPD)P*GP*TP*CP*TP*AP*CP*C)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.72 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 44.906Å b: 94.486Å c: 104.384Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

DNA base damage recognition and removal: new twists and grooves.
Huffman et al. (2005)
18 more

2 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
1 more