› 1l3i

X-ray diffraction
1.95Å resolution

MT0146, THE PRECORRIN-6Y METHYLTRANSFERASE (CBIT) HOMOLOG FROM M. THERMOAUTOTROPHICUM, ADOHCY BINARY COMPLEX

Released:
DOI: 10.2210/pdb1l3i/pdb
PDB entry 1l3i coloured by chain, front view.
PDB entry 1l3i coloured by chain, side view.
PDB entry 1l3i coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
The crystal structure of MT0146/CbiT suggests that the putative precorrin-8w decarboxylase is a methyltransferase.
Keller JP, Smith PM, Benach J, Christendat D, deTitta GT, Hunt JF
Structure 10 1475-87 (2002)
PMID: 12429089

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cobalt-precorrin-6B = S-adenosyl-L-homocysteine + cobalt-precorrin-7 + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-127824 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating) Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 192 amino acids
Theoretical weight: 21.11 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26249 (Residues: 1-192; Coverage: 100%)
Gene names: MTH_146, cbiT
Sequence domains: Methyltransferase domain
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

1 bound ligand:
Ligand SAH 6 x SAH
1 modified residue:
Ligand MSE 48 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: C2
Unit cell:
a: 141.1Å b: 59.4Å c: 165.9Å
α: 90° β: 109.9° γ: 90°
R-values:
R R work R free
0.212 0.212 0.257
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
8 more

5 mentions without citation

Transplanting supersites of HIV-1 vulnerability.
Zhou et al. (2014)
4 more