› 1l3l

X-ray diffraction
1.66Å resolution

Crystal structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA

Released:
DOI: 10.2210/pdb1l3l/pdb
PDB entry 1l3l coloured by chain, front view.
PDB entry 1l3l coloured by chain, side view.
PDB entry 1l3l coloured by chain, top view.
Source organism: Agrobacterium tumefaciens
Primary publication:
Structure of a bacterial quorum-sensing transcription factor complexed with pheromone and DNA.
Zhang RG, Pappas KM, Pappas T, Brace JL, Miller PC, Oulmassov T, Molyneaux JM, Anderson JC, Bashkin JK, Winans SC, Joachimiak A
Nature 417 971-4 (2002)
PMID: 12087407

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114111 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Transcriptional activator protein TraR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 234 amino acids
Theoretical weight: 27.06 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P33905 (Residues: 1-234; Coverage: 100%)
Gene name: traR
Sequence domains:
Structure domains:
5'-D(*GP*AP*TP*GP*TP*GP*CP*AP*GP*AP*TP*CP*TP*GP*CP*AP*CP*AP*TP*C)-3' Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Agrobacterium tumefaciens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand LAE 4 x LAE
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 92.298Å b: 58.37Å c: 127.453Å
α: 90° β: 102.81° γ: 90°
R-values:
R R work R free
0.246 0.246 0.266
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

59 review citations

Quorum sensing: cell-to-cell communication in bacteria.
Waters et al. (2005)
58 more

45 mentions without citation

Quorum sensing signal-response systems in Gram-negative bacteria.
Papenfort et al. (2016)
44 more