› 1l6r

X-ray diffraction
1.4Å resolution

Crystal Structure of Thermoplasma acidophilum 0175 (APC0014)

Released:
DOI: 10.2210/pdb1l6r/pdb
PDB entry 1l6r coloured by chain, front view.
PDB entry 1l6r coloured by chain, side view.
PDB entry 1l6r coloured by chain, top view.
Source organism: Thermoplasma acidophilum
Primary publication:
Structure- and function-based characterization of a new phosphoglycolate phosphatase from Thermoplasma acidophilum.
Kim Y, Yakunin AF, Kuznetsova E, Xu X, Pennycooke M, Gu J, Cheung F, Proudfoot M, Arrowsmith CH, Joachimiak A, Edwards AM, Christendat D
J Biol Chem 279 517-26 (2004)
PMID: 14555659

Function and Biology Details

Reaction catalysed: 
2-phosphoglycolate + H(2)O = glycolate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190900 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycolate phosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 227 amino acids
Theoretical weight: 25.6 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HLQ2 (Residues: 1-224; Coverage: 100%)
Gene name: Ta0175
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 88.24Å b: 99.244Å c: 113.781Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.171 0.195
Expression system: Escherichia coli

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Citations

5 review citations

'Conserved hypothetical' proteins: prioritization of targets for experimental study.
Galperin et al. (2004)
4 more

8 mentions without citation

Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications.
Rao et al. (2006)
7 more