› 1l7a

X-ray diffraction
1.5Å resolution

structural Genomics, crystal structure of Cephalosporin C deacetylase

Released:
DOI: 10.2210/pdb1l7a/pdb
PDB entry 1l7a coloured by chain, front view.
PDB entry 1l7a coloured by chain, side view.
PDB entry 1l7a coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Zhang R, Koroleva O, Collert F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reactions catalysed: 
Cephalosporin C + H(2)O = deacetylcephalosporin C + acetate
Deacetylation of xylans and xylo-oligosaccharides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161105 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cephalosporin-C deacetylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 318 amino acids
Theoretical weight: 35.89 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P94388 (Residues: 1-318; Coverage: 100%)
Gene names: BSU03180, cah
Sequence domains: Acetyl xylan esterase (AXE1)
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R32
Unit cell:
a: 156.446Å b: 156.446Å c: 131.748Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.185 0.189
Expression system: Escherichia coli BL21

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