› 1l7i

X-ray diffraction
1.8Å resolution

Crystal Structure of the anti-ErbB2 Fab2C4

Released:
DOI: 10.2210/pdb1l7i/pdb
PDB entry 1l7i coloured by chain, front view.
PDB entry 1l7i coloured by chain, side view.
PDB entry 1l7i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Comprehensive functional maps of the antigen-binding site of an anti-ErbB2 antibody obtained with shotgun scanning mutagenesis.
Vajdos FF, Adams CW, Breece TN, Presta LG, de Vos AM, Sidhu SS
J Mol Biol 320 415-28 (2002)
PMID: 12079396

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212517 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7Z3Y4 (Residues: 23-236; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
chimera of Fab2C4: "humanized" murine monoclonal antibody Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 41.966Å b: 64.249Å c: 79.445Å
α: 90° β: 105.44° γ: 90°
R-values:
R R work R free
0.197 0.197 0.23
Expression system: Escherichia coli

Quick links

Citations

14 review citations

The molecular architecture of protein-protein binding sites.
Reichmann et al. (2007)
13 more

12 mentions without citation

Matuzumab binding to EGFR prevents the conformational rearrangement required for dimerization.
Schmiedel et al. (2008)
11 more