› 1l8q

X-ray diffraction
2.7Å resolution

CRYSTAL STRUCTURE OF DNA REPLICATION INITIATION FACTOR

Released:
DOI: 10.2210/pdb1l8q/pdb
PDB entry 1l8q coloured by chain, front view.
PDB entry 1l8q coloured by chain, side view.
PDB entry 1l8q coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation.
Erzberger JP, Pirruccello MM, Berger JM
EMBO J 21 4763-73 (2002)
PMID: 12234917

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130359 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosomal replication initiator protein DnaA Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 38.05 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O66659 (Residues: 76-399; Coverage: 81%)
Gene names: aq_322, dnaA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I23
Unit cell:
a: 155.752Å b: 155.752Å c: 155.752Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.229 0.255
Expression system: Escherichia coli

Quick links

Citations

53 review citations

Evolutionary relationships and structural mechanisms of AAA+ proteins.
Erzberger et al. (2006)
52 more

19 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
18 more