› 1l8v

X-ray diffraction
2.8Å resolution

Crystal Structure of a Mutant (C109G,G212C) P4-P6 Domain of the Group I Intron from Tetrahymena Thermophilia

Released:
DOI: 10.2210/pdb1l8v/pdb
PDB entry 1l8v coloured by chain, front view.
PDB entry 1l8v coloured by chain, side view.
PDB entry 1l8v coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Specificity of RNA-RNA helix recognition.
Battle DJ, Doudna JA
Proc Natl Acad Sci U S A 99 11676-81 (2002)
PMID: 12189204

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196102 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
P4-P6 RNA ribozyme domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 157 nucleotides
Theoretical weight: 50.72 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 10 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 80.455Å b: 129.918Å c: 144.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.29 0.29 0.293
Expression system: Not provided

Quick links

Citations

12 review citations

The scanning mechanism of eukaryotic translation initiation.
Hinnebusch AG. (2014)
11 more

8 mentions without citation

m(1)A and m(1)G disrupt A-RNA structure through the intrinsic instability of Hoogsteen base pairs.
Zhou et al. (2016)
7 more