› 1l9e

X-ray diffraction
1.85Å resolution

Role of Histidine 269 in Catalysis by Monomeric Sarcosine Oxidase

Released:
DOI: 10.2210/pdb1l9e/pdb
PDB entry 1l9e coloured by chain, front view.
PDB entry 1l9e coloured by chain, side view.
PDB entry 1l9e coloured by chain, top view.
Source organism: Bacillus sp. B-0618
Primary publication:
Monomeric sarcosine oxidase: role of histidine 269 in catalysis.
Zhao G, Song H, Chen ZW, Mathews FS, Jorns MS
Biochemistry 41 9751-64 (2002)
PMID: 12146941

Function and Biology Details

Reaction catalysed: 
Sarcosine + H(2)O + O(2) = glycine + formaldehyde + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154199 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monomeric sarcosine oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 389 amino acids
Theoretical weight: 43.1 KDa
Source organism: Bacillus sp. B-0618
Expression system: Escherichia coli
UniProt:
  • Canonical: P40859 (Residues: 2-390; Coverage: 100%)
Gene names: sox, soxA
Sequence domains: FAD dependent oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand CL 2 x CL
Ligand IMD 2 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 73.083Å b: 70.638Å c: 74.297Å
α: 90° β: 93.78° γ: 90°
R-values:
R R work R free
0.168 0.168 0.195
Expression system: Escherichia coli

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Citations

1 review citation

Computational tools for the evaluation of laboratory-engineered biocatalysts.
Romero-Rivera et al. (2016)
19 other articles