› 1l9u

X-ray diffraction
4Å resolution

THERMUS AQUATICUS RNA POLYMERASE HOLOENZYME AT 4 A RESOLUTION

Released:
DOI: 10.2210/pdb1l9u/pdb
PDB entry 1l9u coloured by chain, front view.
PDB entry 1l9u coloured by chain, side view.
PDB entry 1l9u coloured by chain, top view.
Source organism: Thermus aquaticus
Primary publication:
Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution.
Murakami KS, Masuda S, Darst SA
Science 296 1280-4 (2002)
PMID: 12016306

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-190208 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, J, K
Molecule details ›
Chains: A, B, J, K
Length: 314 amino acids
Theoretical weight: 34.83 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9KWU8 (Residues: 1-314; Coverage: 100%)
Gene name: rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chains: C, L
Molecule details ›
Chains: C, L
Length: 1118 amino acids
Theoretical weight: 124.8 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9KWU7 (Residues: 1-1119; Coverage: 100%)
Gene name: rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chains: D, M
Molecule details ›
Chains: D, M
Length: 1524 amino acids
Theoretical weight: 171.19 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9KWU6 (Residues: 1-1524; Coverage: 100%)
Gene name: rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chains: E, N
Molecule details ›
Chains: E, N
Length: 99 amino acids
Theoretical weight: 11.64 KDa
Source organism: Thermus aquaticus
UniProt:
  • Canonical: Q9EVV4 (Residues: 1-99; Coverage: 100%)
Gene name: rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chains: H, Q
Molecule details ›
Chains: H, Q
Length: 332 amino acids
Theoretical weight: 38.19 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9EZJ8 (Residues: 92-438; Coverage: 76%)
Gene name: sigA
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 155Å b: 271.2Å c: 155.3Å
α: 90° β: 91.4° γ: 90°
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

73 review citations

Multiple sigma subunits and the partitioning of bacterial transcription space.
Gruber et al. (2003)
72 more

12 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
11 more