› 1l9v

X-ray diffraction
2.6Å resolution

Non Structural protein encoded by gene segment 8 of rotavirus (NSP2), an NTPase, ssRNA binding and nucleic acid helix-destabilizing protein

Released:
DOI: 10.2210/pdb1l9v/pdb
PDB entry 1l9v coloured by chain, front view.
PDB entry 1l9v coloured by chain, side view.
PDB entry 1l9v coloured by chain, top view.
Source organism: Simian 11 rotavirus (serotype 3 / strain SA11-Ramig)
Primary publication:
Rotavirus protein involved in genome replication and packaging exhibits a HIT-like fold.
Jayaram H, Taraporewala Z, Patton JT, Prasad BV
Nature 417 311-5 (2002)
PMID: 12015608

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-structural protein 2 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.62 KDa
Source organism: Simian 11 rotavirus (serotype 3 / strain SA11-Ramig)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03243 (Residues: 1-317; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: I422
Unit cell:
a: 108.432Å b: 108.432Å c: 152.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.287
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Parallels among positive-strand RNA viruses, reverse-transcribing viruses and double-stranded RNA viruses.
Ahlquist P. (2006)
11 more

18 mentions without citation

Conventional and unconventional mechanisms for capping viral mRNA.
Decroly et al. (2011)
17 more