› 1la3

Solution NMR

Solution structure of recoverin mutant, E85Q

Released:
DOI: 10.2210/pdb1la3/pdb
PDB entry 1la3 coloured by chain, ensemble of 14 models, front view.
PDB entry 1la3 coloured by chain, ensemble of 14 models, side view.
PDB entry 1la3 coloured by chain, ensemble of 14 models, top view.
Source organism: Bos taurus
Primary publication:
Structure and calcium-binding studies of a recoverin mutant (E85Q) in an allosteric intermediate state.
Ames JB, Hamasaki N, Molchanova T
Biochemistry 41 5776-87 (2002)
PMID: 11980481

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149260 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Recoverin Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 23.23 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P21457 (Residues: 2-202; Coverage: 100%)
Gene names: RCV1, RCVRN
Sequence domains: EF hand
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand MYR 1 x MYR
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry and simulated annealing
Expression system: Escherichia coli

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Citations

6 review citations

Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling.
Burgoyne RD. (2007)
5 more

2 mentions without citation

Conformational dynamics of recoverin's Ca2+-myristoyl switch probed by 15N NMR relaxation dispersion and chemical shift analysis.
Xu et al. (2011)
1 more