› 1lbm

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF PHOSPHORIBOSYL ANTHRANILATE ISOMERASE (PRAI) IN COMPLEX WITH REDUCED 1-(O-CARBOXYPHENYLAMINO)-1-DEOXYRIBULOSE 5-PHOSPHATE (RCDRP)

Released:
DOI: 10.2210/pdb1lbm/pdb
PDB entry 1lbm coloured by chain, front view.
PDB entry 1lbm coloured by chain, side view.
PDB entry 1lbm coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Two (betaalpha)(8)-barrel enzymes of histidine and tryptophan biosynthesis have similar reaction mechanisms and common strategies for protecting their labile substrates.
Henn-Sax M, Thoma R, Schmidt S, Hennig M, Kirschner K, Sterner R
Biochemistry 41 12032-42 (2002)
PMID: 12356303

Function and Biology Details

Reaction catalysed: 
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-(5'-phosphoribosyl)anthranilate isomerase Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23.07 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q56320 (Residues: 1-205; Coverage: 100%)
Gene names: TM_0139, trpF
Sequence domains: N-(5'phosphoribosyl)anthranilate (PRA) isomerase
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand 137 1 x 137
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELLIOTT GX-20
Spacegroup: P6122
Unit cell:
a: 68.835Å b: 68.834Å c: 187.163Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.17 0.17 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
3 more

6 mentions without citation

Evaluation and ranking of enzyme designs.
Kiss et al. (2010)
5 more