› 1lcd

Solution NMR

STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BASE-PAIR HALF-OPERATOR DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND RESTRAINED MOLECULAR DYNAMICS

Released:
DOI: 10.2210/pdb1lcd/pdb
PDB entry 1lcd coloured by chain, ensemble of 3 models, front view.
PDB entry 1lcd coloured by chain, ensemble of 3 models, side view.
PDB entry 1lcd coloured by chain, ensemble of 3 models, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the complex of lac repressor headpiece and an 11 base-pair half-operator determined by nuclear magnetic resonance spectroscopy and restrained molecular dynamics.
Chuprina VP, Rullmann JA, Lamerichs RM, van Boom JH, Boelens R, Kaptein R
J Mol Biol 234 446-62 (1993)
PMID: 8230225

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114130 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Lactose operon repressor Chain: A
Molecule details ›
Chain: A
Length: 51 amino acids
Theoretical weight: 5.69 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P03023 (Residues: 1-51; Coverage: 14%)
Gene names: JW0336, b0345, lacI
Sequence domains: Bacterial regulatory proteins, lacI family
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*AP*TP*TP*GP*TP*GP*AP*GP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.41 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*CP*GP*CP*TP*CP*AP*CP*AP*AP*TP*T)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.29 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Not provided

Quick links

Citations

8 review citations

The lac repressor.
Lewis M. (2005)
7 more

2 mentions without citation

Type III secretion system effector proteins are mechanically labile.
LeBlanc et al. (2021)
1 more