› 1lcu

X-ray diffraction
3.5Å resolution

Polylysine Induces an Antiparallel Actin Dimer that Nucleates Filament Assembly: Crystal Structure at 3.5 A Resolution

Released:
DOI: 10.2210/pdb1lcu/pdb
PDB entry 1lcu coloured by chain, front view.
PDB entry 1lcu coloured by chain, side view.
PDB entry 1lcu coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Polylysine induces an antiparallel actin dimer that nucleates filament assembly: crystal structure at 3.5-A resolution.
Bubb MR, Govindasamy L, Yarmola EG, Vorobiev SM, Almo SC, Somasundaram T, Chapman MS, Agbandje-McKenna M, McKenna R
J Biol Chem 277 20999-1006 (2002)
PMID: 11932258

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159317 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 371 amino acids
Theoretical weight: 41.34 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 7-377; Coverage: 98%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 4 x CA
Ligand CL 3 x CL
Ligand ATP 2 x ATP
Ligand LAR 2 x LAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 101.456Å b: 103.029Å c: 126.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.266

Quick links

Citations

7 review citations

Molecular architecture of intermediate filaments.
Strelkov et al. (2003)
6 more

4 mentions without citation

Structural basis for the slow dynamics of the actin filament pointed end.
Narita et al. (2011)
3 more